FIG. 4.
EMSA of isolated OF sites. Biotinylated oligonucleotides (50 bp) (see Table S1 in the supplemental material) were tested as described in Materials and Methods using ParG concentrations (μM monomer, left to right): 0, 0.5, 0.75, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0, except for site D from which the 0.75 μM concentration was omitted The oligonucleotides include the 12-bp site cores flanked on either side by 4 bp from the site's normal context (Fig. 1). Unbound DNAs and nucleoprotein complexes are indicated by open and filled arrows, respectively.