FIG. 1.
Growth inhibition kinetics of S. aureus expressing G1ORF67. Clones of S. aureus RN4220 harboring either G1ORF67 or a control ORF (which has no impact on S. aureus growth) under the regulation of an arsenite-regulatable promoter were grown in tryptic soy broth supplemented with 30 μg/ml kanamycin with or without 5 μM NaAsO2. At different time intervals, aliquots of the cultures were plated onto tryptic soy agar plates supplemented with kanamycin in order to determine the number of CFU. Results are expressed as the means ± standard deviations of the results for three independent clones.