TABLE 3.
Effects of hA3G and hA3F on the efficiency of intracellular reverse transcription of a Δvif HIV-1- or HIV-1-derived vector
| Reverse transcription step | Fold change (reduction of products)
|
Reference | |||
|---|---|---|---|---|---|
| WT hA3G | Deaminase-minus hA3G | WT hA3F | Deaminase-minus hA3F | ||
| tRNA priming | 2 (MT2, H9, or 293T) | 1.5 (SupT1) | 79 | ||
| 2 (SupT1 and 293T) | 1.4 (SupT1) | 253 | |||
| Minus-strand strong-stop synthesis | >10 (SupT1) | 2 (SupT1) | 5 (SupT1) | 5 (SupT1) | 96 |
| >20 (SupT1) | 4 (SupT1) | 15 | |||
| 2 (SuptT1) | 79 | ||||
| 2 (SupT1) | 253 | ||||
| 2 (SupT1) | 134 | ||||
| 17. (HeLa) | 1.7 (HeLa) | 2 (HeLa) | 142 | ||
| First-strand transfer | 3 (SupT1) | SupT1: 3-fold | 134 | ||
| 1.15 (293T) | 153 | ||||
| Minus-strand DNA synthesis | 20 (SupT1) | 1.4 (SupT1) | 79 | ||
| 20 (SupT1) | 253 | ||||
| 20 (SupT1) | 134 | ||||
| 3 (HeLa) | 3 (HeLa) | 5 (HeLa) | 142 | ||
| Second-strand transfer | 10 (SupT1) | 10 (SupT1) | >10 (SupT1) | 10 (SupT1) | 134 |
| 2-3 (293T) | 153 | ||||
| Proviral DNA synthesis | 5-10 (293T) | 153 | |||
| 3 (HeLa) | 149 | ||||
| Integration | 5 (293T) | No effect (293T) | 153 | ||
| 3 (HeLa) | 3 (HeLa) | >10 (HeLa) | 142 | ||
| 7 (HOS) | 150 | ||||