Table 1.
S. aureus genes affected by sarZ disruptiona
| ORF | Gene | Fold-change | H2O2 effectb | Description | |
|---|---|---|---|---|---|
| 2 h | 5 h | ||||
| Genes downregulated in the sarZ mutant | |||||
| SACOL1141 | isdC | -2.1 | Iron regulated Surface Determinant system | ||
| SACOL0860 | nuc | -2.5 | - | Thermonuclease precursor | |
| SACOL0459 | pbuX | -2.0 | Xanthine permiase | ||
| SACOL1210 | pyrR | -2.3 | Pyrimidine operon regulatory protein | ||
| SACOL2384 | sarZ | -3.4 | -11.2 | Staphylococcal accessory protein Z | |
| SA0166 | -2.1 | -2.4 | ABC transporter | ||
| SACOL1168 | efb | -2.9 | + | Fibrinogen-binding protein | |
| SACOL1169 | fib | -2.8 | Fibrinogen-binding protein precursor related protein | ||
| SACOL0784 | hisC | -2.0 | Histidinol-phosphate aminotransferase | ||
| SACOL1076 | purS | -2.5 | Phosphoribosylformylglycinamide synthetase | ||
| SACOL2352 | tcaA | -2.4 | Teicoplanin resistance associated protein | ||
| SACOL0157 | -3.0 | α-helical coiled-coil SrpF–like protein | |||
| SA0167 | -2.2 | SrpL–like membrane lipoprotein | |||
| SACOL0159 | -2.2 | ABC transporter | |||
| SACOL0185 | -2.5 | Peptide ABC transporter | |||
| SACOL0186 | -2.7 | Dipeptide ABC transporter, DppC–like protein | |||
| SACOL0218 | -2.0 | + | Conserved hypothetical protein | ||
| SACOL0219 | -2.5 | Conserved hypothetical protein | |||
| SACOL0430 | -2.6 | Cystathionine β-lyase family protein | |||
| SACOL0431 | -2.3 | Cystathionine γ-synthase family protein | |||
| SACOL0505 | -3.1 | ABC transporter | |||
| SACOL0507 | -2.3 | N-acetylmuramoyl-L-Ala amidase precursor | |||
| SACOL0602 | -2.3 | Haloacid dehydrogenase-like protein | |||
| SACOL0883 | -2.7 | ABC transporter | |||
| SACOL0910 | -3.0 | Hypothetical protein | |||
| SACOL1164 | -3.1 | Fibrinogen-binding related protein | |||
| SACOL1996 | -2.4 | ABC transporter | |||
| SACOL1997 | -2.7 | GntR family protein | |||
| SACOL2295 | -3.1 | Staphyloxanthine biosynthesis protein | |||
| SACOL2709 | -2.5 | Conserved hypothetical protein | |||
| SACOL2710 | -2.6 | Conserved hypothetical protein | |||
| Genes upregulated in the sarZ mutant | |||||
| SACOL1783 | acs | 2.2 | - | Acetyl-CoA synthetase | |
| SACOL0964 | argG | 8.2 | Arguininosuccinate synthetase | ||
| SACOL0963 | argH | 5.6 | Arguininosuccinate lyase | ||
| SACOL1429 | asd | 4.4 | Aspartate-semialdehyde dehyrogenase | ||
| SACOL0121 | deoD | 5.2 | + | Purine nucreoside phospholylase | |
| SACOL1872 | epiE | 3.0 | Epidermin immunity protein E | ||
| SACOL1873 | epiF | 2.9 | Epidermin immunity protein F | ||
| SACOL2482 | fabG | 4.1 | + | 3-oxoacyl-ACP reductase | |
| SACOL0655 | fruA | 5.2 | Fructose permiase | ||
| SACOL2525 | gntK | 6.5 | + | Gluconokinase | |
| SACOL2516 | gntR | 5.6 | + | Gluconate operon repressor | |
| SACOL2042 | ilvD | 3.2 | -2.3 | Dihydroxy-acid dehydrates | |
| SACOL2183 | lacD | 2.0 | Tagatose 1, 6-diphosphate aldrase | ||
| SACOL0248 | lrgB | 3.2 | Holin–like protein | ||
| SACOL1428 | lysC | 4.4 | Aspartokinase, α and β subunits | ||
| SACOL1551 | malA | 4.7 | α-glucosidase | ||
| SACOL0192 | msmX | 2.1 | Maltose ABC transporter | ||
| SACOL2397 | nirD | 3.0 | -2.2 | NAD (P) H Nitrite recuctase, small subunit | |
| SACOL1475 | norB | 5.7 | Drug transporter | ||
| SACOL2031 | nrgA | 4.7 | Ammonium transporter family protein | ||
| SACOL0872 | ohr | 2.7 | + | Organic hydroperoxide resistance protein | |
| SACOL1838 | pckA | 2.6 | Posphoenolpyruvate carboxykinase | ||
| SACOL0205 | pflA | 37.3 | + | Pyruvate formate lyase activating enzyme | |
| SACOL0204 | pflB | 18.8 | + | Pyruvate formate lyase | |
| SACOL2376 | scrA | 5.3 | PTS system, Sucrose-specific IIBC component | ||
| SACOL0387 | set11 | 9.1 | Exotoxin 11 | ||
| SACOL0122 | tet38 | 5.1 | + | Tetracycline resistance protein | |
| SACOL0516 | treP | 6.7 | PTS system, IIBC component | ||
| SACOL0517 | treC | 4.0 | α, α phosphotreharase | ||
| SACOL0012 | 2.8 | Homoserin O-acetyltransferase | |||
| SACOL0176 | 6.3 | + | Conserved hypothetical protein | ||
| SACOL0177 | 4.6 | Glucokinase-regulator-related protein | |||
| SACOL0183 | 3.4 | Conserved hypothetical protein | |||
| SACOL0469 | 2.9 | Exotoxin 1 | |||
| SACOL0504 | 6.3 | -3.0 | + | ABC transporter | |
| SACOL0708 | 2.8 | DAK2 domain protein | |||
| SACOL0709 | 3.0 | Conserved hypothetical protein | |||
| SACOL0882 | 2.9 | ABC transporter | |||
| SACOL0884 | 2.1 | -2.3 | ABC transporter | ||
| SACOL1476 | 13.8 | Amino acid permiase | |||
| SACOL1661 | 3.8 | - | Acetyl-CoA carboxylase, accC homologue | ||
| SACOL1847 | 3.8 | Conserved hypothetical protein | |||
| SACOL1849 | 2.3 | - | Conserved hypothetical protein | ||
| SACOL1915 | 4.1 | Glutamate ABC transporter | |||
| SACOL1916 | 3.8 | Amino acid ABC transporter | |||
| SACOL2461 | 3.7 | + | Conserved hypothetical protein | ||
| SACOL2579 | 3.6 | Phytone dehydrogenase | |||
| SACOL2585 | 4.0 | -2.0 | pfoS/R–like regulatory protein | ||
| SACOL2619 | 3.1 | -2.4 | Amino acid permiase | ||
| SACOL2704 | 3.0 | ATP phosphoribosyltransferase reguratory subunit | |||
| SAS0383 | 2.2 | Exotoxin | |||
| SAV0894 | 2.2 | ϕMu50B-like protein | |||
| SACOL0212 | 2.1 | 3-hydroxyacyl-CoA dehydrogenase | |||
a
The entire microarray data were deposited to the National Center for Biotechnology Information Gene Expression Omnibus (NCBI GEO, http://www.ncbi.nlm.nih.gov/geo/) with a provisional entry number GSE13138. Three uncharacterized ORFs were omitted in this table.
b
Previously described ORFs affected by exposure to hydrogen peroxide (Chang et al., 2006a).
+, Upregulation. -, Downregulation.