Skip to main content
. Author manuscript; available in PMC: 2009 Jun 18.
Published in final edited form as: Brain Res. 2008 Nov 5;1249:244–250. doi: 10.1016/j.brainres.2008.10.055

Fig. 2.

Fig. 2

LY379268 induces the phosphorylation of ERK (Thr202/Tyr204). A primary cortical neuron culture (DIV 7–10) was treated with 10 µM LY379268 for indicated time and then the extracted proteins were subjected to immunoblot with phospho-ERK (Thr202/Tyr204) specific antibody. The phosphorylation level is increased after 10 min treatment and gradually decreased to basal level. The immunoblot with an antibody, which detects total ERK level, indicates that the total protein level of ERK is not changed by LY379268 treatment. The level of β-actin was used to normalize total ERK and phospho-ERK level. Data in the graph represent means ± SD values from triplicate samples from at least three separate experiments.