Table 2.
Summary of metabolic activities of SEAP-producing CHO cells in response to temperature reduction
| Culture conditions | qGlC (μmol/106 cells/h) | qLac (μmol/106 cells/h) | YLac/Glc (mol/mol) | qGln (nmol/106 cells/h) | qAmm (nmol/106 cells/h) | YAmm/Gln (mol/mol) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Beforea | Afterb | Before | After | Singlec | Before | After | Before | After | Single | |||
| Before | After | Before | After | |||||||||
| Suspension | 0.28 | 0.12 | 0.51 | 0.17 | 1.7 | 18 | 1.2 | 17 | 1.4 | 1.1 | ||
| 1.82 | 1.42 | 0.95 | 1.2 | |||||||||
| Microcarrier (Cytodex 3) | 0.32 +14%d |
0.07 −42% |
0.67 +31% |
0.13 −24% |
1.9 | 35 +89% |
7.8 +550% |
29 +66% |
11 +655% |
1.0 | ||
| 2.09 +15% |
1.82 +28% |
0.83 −13% |
1.4 +16% |
|||||||||
| Microcarrier (Cytopore 1) | 0.24 −14% |
0.10 −17% |
0.47 −8% |
0.12 −29% |
1.9 | 29 +57% |
11 +870% |
27 +54% |
13 +804% |
0.9 | ||
| 1.96 +8% |
1.20 −16% |
0.93 −2% |
1.1 −7% |
|||||||||
aBefore low temperature shift
bAfter low temperature shift
cSingle-temperature cultures
dPercent change compared to suspension culture