Table 4.
Summary of the decontamination techniques used during the development of tissue culture method for the zebra mussel and their effect on cell viability
| Disinfectant | Disinfection | Cell viability | ||
|---|---|---|---|---|
| <30 sec | 60 sec | <30 sec | 60 sec | |
| Bleach | ||||
| 5% | − | − | + | − |
| 10% | − | + | + | − |
| 20% | − | + | − | − |
| Ethanol 70o | − | + | + | − |
| Initial anti-b | <10 min − |
>15 min + |
<10 min + |
>15 min − |
| Final anti-b (X4, X2, X1) |
10 min − |
30, 20, 10 min + |
10 min + |
30, 20, 10 min +++ |
| DTT | Whole tissue + |
Cut tissue + |
Whole tissue + |
Cut tissue + |
For cell viability: −, ≤ 40%; +, 41–60%; +++, >81% of cells in field of view (×400 magnification). For disinfection: −, infected with bacteria; +, no bacteria present. Anti-b, antibiotic solution