Table 6.
Summary of different methods of tissue dissociation and their effect on cell viability on tissues of the zebra mussel in culture
| Dissociation method | Dissociation | Cell viability | ||
|---|---|---|---|---|
| No dissociation | − | − | ||
| Collagenase | 6 mg/ml PBS + |
5 mg/ml trypsin + |
6 mg/ml PBS + |
5 mg/ml trypsin − |
| Trypsin | 2.5% in PBS + |
0.025% in media + |
2.5% in PBS − |
0.025% in media − |
| EDTA | 5 mM + |
5 mM + |
||
| Pronase | 0.025% in buffer ++ |
0.025% in buffer ++ |
||
−, ≤40%; +, 41–60%; ++, 61–80% of cells in field of view (×400 magnification) for tissue dissociation and cell viability