FIG. 3.

Phenotypic characterization of the SMU.1297 deletion mutants. Overnight cultures of the wild type (UA159), the ISS1 insertion mutant (IBS921), the clean Cre-loxP deletion mutant (IBS934), and the complemented strain (IBS934/pIB1297) were grown in THY medium with appropriate antibiotics. Different dilutions of the overnight culture, with a starting optical density (A₆₀₀) of 5.0 made in 0.85% NaCl, were spotted on THY agar (THY) or THY agar supplemented with 5 mM MV (THY+MV) or 25 μg/ml menadione (THY+MND) and incubated overnight under microaerophilic conditions at 37°C. Experiments were repeated at least three times, and relevant areas of the representative plates are shown.