Genomic context and sequence alignment of SMU.1297. (A) Organization of open reading frames in the SMU.1297 region in various members of the Firmicutes. The SMU.1297 locus (black arrow) appears to be variable in different Firmicutes. However, the gene immediately downstream of SMU.1297, rpmE, appears to be conserved in streptococci. A putative GST gene (dark gray arrows) is located immediately upstream of SMU.1297 in S. mutans and S. thermophilus only. Smu. S. mutans; Sag, Streptococcus agalactiae; Spr, Streptococcus pneumoniae; Spy, S. pyogenes; Bsu, B. subtilis; Str, S. thermophilus; Sgo, Streptococcus gordonii; Lac, Lactococcus lactis; Efa, Enterococcus faecalis; and Sau, S. aureus. (B) Comparison of the amino acid sequence of SMU.1297 with homologous proteins identified by BLASTP search. Clustal-W was used to align the sequences from different Firmicutes.