TABLE 2.
Enzymatic activities in a wild-type and a ppk ppx mutant E. coli strainc
| Length of culture (h)a | Rate of MTTb reduction (nmol min−1 mg protein−1)
|
Rate of H2O2 decomposition (μmol min−1 mg protein−1)
|
||||
|---|---|---|---|---|---|---|
| NADH dehydrogenase-2
|
Succinate dehydrogenase
|
|||||
| MC4100 | LSB022 | MC4100 | LSB022 | MC4100 | LSB022 | |
| 7 | 1,284 ± 65 | 1,230 ± 68 | 602 ± 22 | 611 ± 44 | 16 ± 3 | 15 ± 1 |
| 48 | 1,257 ± 54 | 257 ± 48 | 624 ± 28 | 188 ± 21 | 26 ± 1 | 14 ± 2 |
a
Cells were grown in MT+P.
b
MTT, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide.
c
Values are averages ± standard deviations.