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Biochemical properties of recombinant Stp1. (A) SDS-PAGE analysis of purified Stp1 after being stained with Coomassie blue. Molecular mass standards are indicated on the left. (B) Dephosphorylation of Stk1 by StP1. Purified Stk1 phosphorylated in vitro with [γ-32P]ATP was incubated without (top) or with (bottom) Stp1 for the indicated times. Equal amounts of Stk1 were analyzed by electrophoresis, and radioactivity was revealed by autoradiography.
