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. 2009 Apr 17;191(13):4427–4440. doi: 10.1128/JB.00103-09

FIG. 11.

FIG. 11.

Orientation of the type 1 fimbria IE in strains BEN2908, BEN2908 ΔORF1frz::kan, and BEN2908 ΔORF5-8frz::kan. (A and B) Strain BEN2908 (lanes 2) and its isogenic deletion mutants BEN2908 Δfrz (lanes 3), BEN2908 ΔORF1frz::kan (lanes 4), and BEN2908 ΔORF5-8frz::kan (lanes 5) were grown without agitation to an OD600 of 1 (A) or for 24 h (B) in LB medium containing no antibiotic. The IEs from both phase-ON and phase-OFF bacteria were then amplified by PCR. The amplified fragments were hydrolyzed with SnaB1, and the digested products were separated on a 2% agarose gel. Lanes 1 and 6 contain molecular weight standards of 200, 400, and 600 bp.