FIG. 1.

Regulatory region of the RSP3361 gene. The numbered nucleotides in the intergenic region between the RSP3360 gene and the RSP3361 gene are shown. Both presumptive PrrA sites are indicated, with their respective half-sites (1 and 2) marked by dark-shaded boxes. The size of half-site 2 is increased from 5 to 6 nt, and the data justifying that change are shown in the accompanying paper (28a). The putative σ⁷⁰ promoter binding sequence is indicated in boldface type, above the sequence, and the matching nucleotides in the −35 and −10 regions are underlined. DNA regions with intrinsic bending propensity are highlighted by light-shaded boxes. Areas requiring higher energy to melt, according to SIDD analysis, are denoted by thick lines above the sequences, whereas areas requiring low energy are shown by thick lines below the sequences. The direction of transcription for both genes is indicated by arrows. The RSP3360 gene, encoding a putative adenine-specific methyltransferase, is transcribed in the same direction as is the RSP3361 gene, but is not PrrA regulated (29).