TABLE 3.
Genes with altered expression profiles in the Δcrp mutant compared to the wild type for cells grown under O2-limited conditions
| Locus | Gene | Function or description | Δcrp mutant/wild type ratioa |
|---|---|---|---|
| Upregulated genes | |||
| Heme biosynthesis | |||
| SO_0027 | hemG-2 | Protoporphyrinogen oxidase | 0.05 |
| SO_0435 | hemE | Uroporphyrinogen decarboxylase | 0.22 |
| SO_2109 | hemH-1 | Ferrochelatase | 0.34 |
| SO_2587 | hemB-1 | Delta-aminolevulinic acid dehydratase | 0.20 |
| SO_3720 | hemG-3 | Protoporphyrinogen oxidase | 0.30 |
| SO_4208 | hemB-2 | Delta-aminolevulinic acid dehydratase | 0.25 |
| Respiratory functions | |||
| SO_0396 | frdC | Quinol:fumarate reductase | 0.13 |
| SO_0848 | napA | Periplasmic nitrate reductase | 0.30 |
| SO_0902 | nqrA | Na-translocating NADH-quinone reductase subunit A | 0.21 |
| SO_0903 | nqrB | Na-translocating NADH-quinone reductase subunit B | 0.14 |
| SO_0904 | nqrC | Na-translocating NADH-quinone reductase subunit C | 0.11 |
| SO_0970 | fccA | Periplasmic fumarate reductase | 0.14 |
| SO_2912 | pflB | Pyruvate formate lyase | 0.17 |
| SO_2913 | pflA | Pyruvate formate lyase 1 activating subunit | 0.20 |
| SO_3922 | fdh | Formate dehydrogenase, cytochrome b | 0.32 |
| SO_4591 | cymA | Membrane-anchored tetraheme c cytochrome | 0.22 |
| Regulatory functions | |||
| SO_0490 | Transmembrane transcriptional regulator | 0.19 | |
| SO_0940 | Transmembrane transcriptional regulator | 0.07 | |
| SO_1415 | Transcriptional regulator, TetR family | 0.12 | |
| SO_3059 | Sigma 54 transcriptional regulator, Fis family | 0.28 | |
| SO_3538 | hlyU | Transcriptional regulator | 0.06 |
| SO_3627 | Transcriptional regulator, TetR family | 0.14 | |
| Downregulated genes | |||
| SO_0852 | Type IV pilin, putative | 4.76 | |
| SO_1329 | cyaC | Adenylate cyclase | 2.08 |
| SO_4312 | cyaA | Adenylate cyclase | 3.97 |
| SO_4609 | coxC | aa3-type cytochrome c oxidase, subunit III | 2.68 |
a
Ratio of the mean hybridization signal intensity for the crp mutant to the mean hybridization signal intensity for the wild type. Values greater than 1 indicate an increase in the mRNA level in the mutant; values less than 1 indicate a decrease.