TABLE 4.
RT-PCR confirmation of microarray data
| Gene product | Gene | N315 ORF | DNA microarray expressiona | RT-PCR expressionb |
|---|---|---|---|---|
| Phosphoribosylpyrophosphate amidotransferase | purF | SA0922 | −3.0 | −3.0 |
| Phosphoribosylaminoimidazole carboxamide formyltransferase | purH | SA0925 | −1.8 | −2.0 |
| Autolysis and methicillin resistance-related protein | fmtA | SA0909 | 2.3 | 3.5 |
| Hypothetical protein, similar to penicillin-binding protein 1A/1B | sgtB | SA1691 | 2.5 | 4.0 |
| Holin-like protein | lrgA | SA0252 | 2.8 | 3.0 |
| Peptidyl-prolyl cis/trans-isomerase homolog | prsA | SA1659 | 2.9 | 3.4 |
| 1-Pyrroline-5-carboxylate dehydrogenase | rocA | SA2341 | 3.5 | 9.0 |
| Ornithine aminotransferase | rocD | SA0818 | 2.5 | 3.0 |
| Vancomycin resistance-associated two-component sensor histidine kinase | vraS | SA1701 | 2.5 | 10.0 |
a
Expression of genes was filtered by using a change of >1.5-fold and a false discovery rate of <1.5%. Negative values indicate downregulation in the ΔpknB mutant strain compared to isogenic wild-type strain S. aureus 8325. Positive values indicate upregulation in the ΔpknB mutant.
b
Expression levels were analyzed by RT-PCR. Negative values indicate downregulation in the ΔpknB mutant strain compared to isogenic wild-type strain S. aureus 8325. Positive values indicate upregulation in the ΔpknB mutant.