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. 2009 Apr 24;191(13):4465–4472. doi: 10.1128/JB.01729-08

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FIG. 2. — Glycolipid profiles of C. glutamicum, C. glutamicum ΔpimB′, C. glutamicum ΔmgtA, C. glutamicum ΔpimB′ ΔmgtA, C. glutamicum ΔpimB′ ΔmgtA-pEKEx2-Rv2188c, and C. glutamicum ΔpimB′ ΔmgtA-pEKEx3-Rv0557. The polar lipid extracts were examined by 2D-TLC on aluminum-backed plates of silica gel 60 F₂₅₄ (Merck 5554), using CHCl₃/CH₃OH/H₂O (60:30:6, vol/vol/vol) in the first direction and CHCl₃/CH₃COOH/CH₃OH/H₂O (40:25:3:6, vol/vol/vol/vol) in the second direction. Glycolipids were visualized by spraying plates with α-naphthol/sulfuric acid, followed by gentle charring of the plates.