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. 2009 Apr 24;191(13):4465–4472. doi: 10.1128/JB.01729-08

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FIG. 3. — MALDI-TOF MS analyses of glycolipids from C. glutamicum, C. glutamicum ΔpimB′, C. glutamicum ΔmgtA, C. glutamicum ΔpimB′ ΔmgtA, C. glutamicum ΔpimB′ ΔmgtA-pEKEx2-Rv2188c, and C. glutamicum ΔpimB′ ΔmgtA-pEKEx3-Rv0557. (A) Negative-ion-mode MALDI-TOF MS analysis of total glycolipid extract from strains. The peaks observed are m/z 836 (M-H)⁻ [PI with C₁₆/C_18:1 fatty acyl groups], m/z 998 (M-H)⁻ [PIM₁ with C₁₆/C_18:1 fatty acyl groups], m/z 1236 (M-H)⁻ [Ac₁PIM₁with 2C₁₆/C_18:1 fatty acyl groups], and m/z 1,398 (M-H)⁻ [Ac₁PIM₂ with 2C₁₆/C_18:1 fatty acyl groups]. The peak m/z 748 was not attributable to any PIM species and, as such, may represent unidentified lipid species and/or plasticizer. (B) Positive-ion MALDI-TOF MS spectrum of the cationized, sodiated precursor ion (M-H + 2Na)⁺ of GlcAGroAc₂ and ManGlcAGroAc₂ at m/z 815 and m/z 977, respectively.