Skip to main content
. 2000 Jul 11;97(15):8548–8553. doi: 10.1073/pnas.140210597

Figure 2.

Figure 2

Properties of LMB-2 molecules. (A) SDS/PAGE analysis of PEGylated LMB-2s. SDS/PAGE was performed under non-reducing conditions. Lanes: M, molecular weight standards from top to bottom: 111,000, 73,000, 47,500, 33,900, 28,800, and 20,500; 1, LMB-2; 2, cys1-LMB-2; 3, PEG5k-LMB-2; 4, PEG20k-LMB-2. (B) In vitro specific cytotoxicity of various forms of LMB-2 on ATac4 cells. Various LMB-2s were diluted with 0.2% BSA in DPBS. ATac-4 cells were seeded at 2.0 × 104 cells/well in 96-well plates 24 h before the addition of LMB-2 (○), cys1-LMB-2 (●), PEG5k-LMB-2 (□), PEG20k-LMB-2 (■) and were incubated with them at 37°C for 24 h, and then were assayed by measuring inhibition of incorporation of 3H-leucine.