Figure 5.

Pharmacokinetics of PEGylated LMB-2s in mice. Normal female BALB/c mice were injected intravenously with 2 μg of LMB-2 (○), cys1-LMB-2 (●), PEG5k-LMB-2 (□), PEG20k-LMB-2 (■). Blood samples were drawn at different times. The level of immunotoxin was measured by bioassay in which diluted serum samples were incubated with Atac-4 cells, and the ability of serum samples to inhibit protein synthesis was measured. A standard curve was made for each immunotoxin. Groups of four mice were used. Values are mean ± SD.