FIG. 4.

Organization of a benzoate-induced gene cluster obtained from a cosmid gene library of D. multivorans. A gene probe deduced from the conserved region of benzoate-CoA ligase was used to screen the cosmid gene library. The open reading frames bcl (1,551 bp) and acd (1,173 bp) were sequenced completely, while rre (>688 bp) and gcd (>1,118 bp) were partially sequenced. Assignments of the putative open reading frames to gene products are as follows: rre, DNA-binding response regulator; bcl, benzoate-CoA ligase; gdh, glutaryl-CoA dehydrogenase/acyl-CoA dehydrogenase; gcd, Na⁺-pumping decarboxylase, β-subunit. Bars 1 to 4 indicate the regions of the gene cluster, which were amplified using cDNA obtained from total RNA using appropriate oligonucleotide primers. The corresponding amplificates were obtained only with cDNA from cells grown on benzoate but not with cDNA from cells grown on cyclohexane carboxylate or lactate.