TABLE 1.
Properties of wild-type and recombinant GDHGeo and recombinant GDHDesa
| Potein | Substrate | Product(s) | Molecular mass (kDa) | Mean native mass (kDa) ± SD | Suggested composition | Mean Km of glutaryl-CoA (μM) ± SD | Mean Vmax (μmol min−1 mg−1) ± SD | Absorption maxima (nm) | No. of FADs/monomer | Inhibitor |
|---|---|---|---|---|---|---|---|---|---|---|
| GDHGeo WT | Glutaryl- CoA | Crotonyl-CoA, CO2 | 43.1 | 170 ± 10 | α4 | 30 ± 2 | 3.2 ± 0.2 | 370, 450 | 0.6 | None |
| GDHGeo RC | Glutaryl | Crotonyl-CoA, CO2 | 43.1 | 180 ± 10 | α4 | 31 ± 2 | 3.6 ± 0.2 | 369, 448 | 0.62 | None |
| GDHDes RC | Glutaryl | Glutaconyl-CoA | 43.4 | 180 ± 10 | α4 | 52 ± 5 | 11 ± 1 | 370, 447 | 0.65 | Glutaconyl-CoA Ki of 5-10 μM |
a
The molecular masses deduced from the amino acid sequence are presented. WT, wild type; RC, recombinant.