FIG. 2.

Northern blotting analysis of the stress-inducible expression of the adhA, sll1106, and sll1107 genes. Synechocystis sp. strain PCC 6803 cells at the exponential phase of growth were subjected to various conditions, and total RNA was extracted at different time points. Fifteen micrograms of total RNA was denatured, separated by electrophoresis in a 1.2% agarose gel, blotted, and hybridized with probes for the adhA, sll1106, and sll1107 genes. The filters were stripped and hybridized with an rnpB gene probe as a loading control. All probes were synthesized as described in Materials and Methods. Three independent experiments were performed, and data plotted are the averages ± standard errors.