TABLE 3.
Kinetic parameters of AdhA from Synechocystisa
| Substrate | Mean Km (mM) ± SDa | Mean kcat (min−1) ± SD | kcat/Km ratio (min−1 mM−1) |
|---|---|---|---|
| Acetaldehyde | 0.510 ± 0.050 | 9,458 ± 138 | 18,545 |
| Butanal | 0.110 ± 0.008 | 9,398 ± 181 | 84,664 |
| Pentanal | 0.075 ± 0.002 | 9,547 ± 185 | 123,295 |
| Cinnamaldehyde | 0.017 ± 0.001 | 6,475 ± 131 | 380,882 |
| Ethanol | 18.800 ± 3.300 | 1,641 ± 33 | 87 |
| Butanol | 3.280 ± 0.100 | 1,023 ± 21 | 312 |
| Pentanol | 1.540 ± 0.060 | 789 ± 15 | 512 |
| Cinnamyl alcohol | 0.270 ± 0.010 | 3,647 ± 73 | 13,507 |
| NADPH | 0.025 ± 0.007 | ||
| NADP | 0.050 ± 0.007 | ||
| NADH | 1.310 ± 0.060 | ||
| NAD | 9.700 ± 0.800 |
a
Reactions were performed using 30 mM Tris-HCl (pH 8.0). For the reduction of aldehydes and oxidation of alcohols, 0.15 mM NAD(P)H and 0.4 mM NAD(P)+ were used, respectively. Kinetic constants for NAD(P)+ and NAD(P)H were determined with 100 mM ethanol and 25 mM acetaldehyde, respectively.