FIG. 2.

Northern blot analysis of clhAB₂ transcription. B. anthracis Sterne, its lytSR mutant derivative (lytSR), and the lytSR complementation strain (comp) were grown in the presence (+Glu) or absence (−Glu) of 35 mM glucose, and RNA was collected at 2, 6, and 12 h. Portions (5 μg) of samples were separated in a 1.0% formaldehyde-agarose gel, transferred to a nylon membrane, and hybridized to DIG-labeled probes derived from the clhB₂ gene. An ethidium bromide-stained gel of the RNA used in these experiments is also shown as a loading control.