TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype(s) or phenotype(s) | Source or reference |
|---|---|---|
| S. sanguinis strains | ||
| SK36 | Human plaque isolate | 37 |
| JFP36 | Emr SSA_0169::pSerm; derived from SK36 | 68a |
| JFP76 | Tetr SSA_0169::tetM; derived from SK36 | 68a |
| JFP57 | Spcr CmrssaB::mariner2 SSA_0169::SSA_0169::ssaB-aad9 | This study |
| JFP58 | Spcr Cmr EmrssaB::mariner2/pJFP58 | This study |
| 0588k | Kanr SSA_0588::aphA-3 | This study |
| Plasmids | ||
| pVA838 | Emr; E. coli-Streptococcus shuttle plasmid | 43 |
| pJFP46 | Kanr SSA_0169::SSA_0169 locus with AscI-NotI-NcoI adapter | 68a |
| pJFP57 | Kanr Spcr; pJFP46 containing ssaB-aad9 | This study |
| pJFP58 | Emr Spcr; pVA838 containing ssaB-aad9 | This study |
| pR412 | Spcr; source of aad9 gene | 45 |