FIG. 4.

VP35 inhibits type I IFN transcription without requiring DLC8 binding. Murine L and NIH 3T3 cells were transfected with wild-type or mutant VP35 along with the pGL4 reporter driven by the IFN-β promoter and pRL-TK reporters. Eight hours posttransfection, cells were infected with Sendai virus for 16 h. Luciferase activities were normalized by Renilla luciferase activity, and reporter activities in infected cells are expressed relative to those in mock-infected cells. Values represent the average of three determinations ± standard deviation.