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. 2009 Apr 22;83(13):6610–6623. doi: 10.1128/JVI.02655-08

FIG. 2.

FIG. 2.

Interaction of pUL25 with nucleoporins. Vero cells cotransfected with plasmids expressing myc-tagged nucleoporins, as described in the legend of Fig. 1, and either HA-pUL25 or HA-pUL32 were lysed 15 h after transfection. Following immunoprecipitation with anti-myc antibody A14, cell extracts (top) and immune complexes (middle and bottom) were separated by SDS-PAGE and analyzed by Western blotting using anti-HA MAb F7 to reveal the presence of HA-pUL25 (left top and left middle) and HA-pUL32 (right top and right middle). The presence of the nucleoporins in the immunoprecipitate was confirmed by stripping the blots and reprobing with anti-myc MAb 9E10 (bottom). The positions of the HA-pUL25 and HA-pUL32 bands and of protein size standards are indicated to the left and right, respectively. UP indicates an unknown protein in the cell extracts that cross-reacts with anti-HA MAb F7. The relative amount of protein present in the immunoprecipitate (IP) is indicated as a percentage of the amount present in the cell extract (CE) (IP:CE ratio).