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CAN/Nup214 depletion delays HSV-1 gene expression. HeLa cells infected with lentiviruses expressing shRNA directed against the GFP gene (shGFP) or CAN/Nup214 (shCAN) were infected with 5 PFU/cell of tsK/Luci. After incubation at 31°C for 2 h, 3 h, or 7 h, the cells were harvested and processed for luciferase assays. The luciferase activity is indicated on a logarithmic-scaled graph, with the numerical value indicated above each bar. pi, postinfection.
