FIG. 6.

ICP4-CFP expression and localization in shLuc and shCAN cells. (A) shLuc or shCAN cells were transfected with a plasmid encoding ICP4-CFP. Twenty-four hours later, cells were fixed, and ICP4-CFP localization was assessed by direct CFP fluorescence. Nuclei were counterstained with DAPI. Scale bars, 5 μm. (B) The fluorescence level of nine randomly chosen CFP-positive cells per condition (shLuc or shCAN), imaged using the same acquisition parameters, was quantified on a scale from 0 (no fluorescence) to 250 (saturated fluorescence). The values obtained were averaged and compared on a graph.