TABLE 1.
PCR primers used to generate DNA fragments used in interaction studiese
| Construct (residues) | Templatea | PCR primerf |
|---|---|---|
| myc-UL36 (2037-3164) | cos14 | CGCGAATTCGATGTGTCCCGAGGCGGCA* |
| CGATCTAGACTAGCCCAGTAACATGCGCAC | ||
| myc-UL36 (2037-2572) | cos14 | CGCGAATTCGATGTGTCCCGAGGCGGCA* |
| AATTCTAGACTACATGCGAGGGGGGACGGG | ||
| myc-UL36 (2037-2504) | cos14 | CGCGAATTCGATGTGTCCCGAGGCGGCA* |
| GGATCTAGACTACGGCCGCCCGAGGATAGC | ||
| myc-UL36 (2037-2353) | cos14 | CGCGAATTCGATGTGTCCCGAGGCGGCA* |
| CGCTCTAGACTAGGGCGCGCCCAGGAGCAC | ||
| myc-UL36 (2037-2200) | cos14 | CGCGAATTCGATGTGTCCCGAGGCGGCA* |
| GCCTCTAGACGGAGGGCCTCCGGCCG | ||
| myc-UL36 (2446-3164) | cos14 | GGAGAATTCCATGACCCCGGTCGCGGTG* |
| CGATCTAGACTAGCCCAGTAACATGCGCAC | ||
| myc-UL36 (2446-3104) | cos14 | GGAGAATTCCATGACCCCGGTCGCGGTG* |
| TGCTCTAGAGGGCGGGGGGCCGAATTG | ||
| myc-UL36 (2446-2986) | cos14 | GGAGAATTCCATGACCCCGGTCGCGGTG* |
| ATATCTAGAGGGTGCTACATGCCCG | ||
| myc-hNup133 | pLex12-hs133b | CGCGAATTCGGGTACCGGGTCCCGAAGG* |
| TAGCTCGAGTTATATTTGTCCCTGAACATA | ||
| myc-hNup50 | IMAGE clone 6463796 | GAAGCGGCCGCATGGCCAGTGAGGAAGTC |
| TTTTCTAGATCAGGCATCCTTTTTCTCC | ||
| myc-hNup54 | IMAGE clone 3931652 | CTTCTCGAGCATGGCCTTCAATTTTGGG* |
| TGTCTCGAGTCAACTAAAGACACCACC | ||
| myc-hCAN Ct | psRET-CAN Ctc | CTCGAATTCTGCAGCTGTCACAGCAGCT* |
| GACCTCGAGTCAGCTTCGCCAGCC | ||
| myc-UL6 HSV | pAC-UL6 HSV1d | GGCGAATTCGACCGCACCACGCTCGCG* |
| GGCTCTAGATCATCGTCGGCCGTC |
a
cos14 is described in reference 12.
b
Provided by V. Doye (4).
c
Provided by M. Fornerod (56).
d
Provided by F. Homa (34).
e
The PCR primers are listed in pairs, with the forward primer listed first and the reverse primer second. All PCR products were cloned into pCDNA3.1-myc, and the restriction enzyme sites used for cloning are underlined.
f
* indicates that these primers have a +1 nucleotide after the restriction site to put the gene in frame with the tag.