TABLE 3.
Yeast two-hybrid interactions between PrV UL25 and a selection of nucleoporin constructs
| Gene (residues)a | β-Gal activityb
|
||
|---|---|---|---|
| HIV-1 Revc | UL25 PrVc | LexA alone | |
| CAN/Nup214 (1-256) | − | − | − |
| CAN/Nup214 (586-1059) | − | − | − |
| CAN/Nup214 FG | + | − | − |
| hCAN Ct (CAN/Nup214 Ct) (1594-2090) | NT | + | 0 |
| POM121 (73-801) | − | − | − |
| POM121 FG | + | − | − |
| Nup98 FG | + | − | − |
| hCG1 Full length (1-423) | 0 | 0 | 0 |
| hCG1 (1-380) | + | + | − |
| hCG1 FG | + | − | − |
| hCG1 Nt | − | − | − |
| Nup153 full length | + | − | − |
| Nup153 Nt | − | − | − |
a
Cloned into vector pGAD, fusing the GAL4-activating domain to the N terminus of the indicated protein. These plasmids were described previously in reference 27, except for hCAN Ct, which contains the C-terminal region of CAN/Nup214, encoding amino acids 1594 to 2090, which was also present in the myc-hCAN Ct plasmid used in the immunoprecipitation studies (Fig. 1 and 2).
b
− indicates no interaction, + indicates an interaction, and 0 indicates poor yeast growth. NT, not tested.
c
Cloned into vector pLexA, fusing the LexA domain to the N terminus of the indicated protein.