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. 2009 Apr 22;83(13):6554–6565. doi: 10.1128/JVI.02550-08

FIG. 2.

FIG. 2.

Cyclosporine alters the subcellular location of NS5B and reduces levels of PK-resistant NS5B within replication complexes. (Aa and e) Cytoplasmic and punctuate localization of NS5B in untreated replicon cells. (b and f) Cyclosporine (CsA) treatment results in a diffused staining pattern for total NS5B. (c and g) PK digestion removes nonprotected NS5B, revealing perinuclear and reticular staining. (d and h) Cyclosporine reduces the accumulation of NS5B in the perinuclear area as well as the staining intensity of PK-resistant NS5B. (Ba to e) Colocalization of nascent HCV RNA and NS5B in PK-resistant, dot-like structures around the nucleus. (f to j) Cyclosporine inhibits the synthesis of nascent RNA and the incorporation of NS5B into PK-resistant replication complexes. DNA was stained with DAPI.