FIG. 5.
Effect of cyclosporine on PK resistance of NS5B and membrane enrichment of CyPA in HCV-infected cells. (A) PK-resistant NS5B in JFH-1-infected cells exhibits cyclosporine (CsA) sensitivity. Huh-7.5 cells that were infected with HCVcc were treated with or without cyclosporine for 22 h and then processed as described in the legend of Fig. 1. Results for treatment with 0.25 μg/ml PK are shown. (B) The level of membrane association of CyPA is increased in the infected cells in a cyclosporine-sensitive manner. Proteins isolated from 4 × 105 cells were loaded into each lane. (C) Quantitative results for of three independent experiments similar to the one shown above (B). The Western blotting bands were quantified using Quantity One software with a Bio-Rad gel documentation system (see Materials and Methods).