TABLE 2.
Quantitative PCRs for the 5′ UTR and E2 regions of HCVa
| Region for quantification | No. of copies/ml
|
5′ UTR/E2 ratio | |
|---|---|---|---|
| 5′ UTR | E2 | ||
| R2 | 2.0 × 106 | 1.7 × 106 | 1.17 |
| R4 | 5.3 × 106 | 2.2 × 106 | 2.44 |
| T5 | NDb | NDb | |
| K1 | 8.3 × 105 | 8.4 × 105 | 0.99 |
| K2 | 3.6 × 105 | 3.5 × 105 | 1.01 |
| K3 | 8.6 × 105 | 5.1 × 105 | 1.7 |
| K4pre | 8.1 × 105 | 3.3 × 105 | 2.45 |
| K4af24 | 4.5 × 105 | 4.4 × 105 | 1.01 |
a
For quantification of the 5′ UTR and E2 regions, the TaqMan Fast PCR Universal mixture and the 7500 Fast Real-Time PCR system (Applied Biosystems) were used in a two-step method with the primers and probes shown in Table 1 according to the manufacturer's protocol. The copy number of HCV was determined by the standard-curve method with serial dilutions of the synthesized full-length HCV RNA.
b
ND, not determined due to sample shortage.