FIG. 1.

Induction of cytokines by virus infection. (A) Confluent HeLa cells in six-well plates were either mock infected or infected with vvΔE3L-Rev or vvΔE3L at an MOI of 5 and collected at the times indicated. Select wells were also treated with 50 μg/ml of araC 1 h prior to infection. Cytokine expression was analyzed by RT-PCR. (B) Confluent BHK21 cells were infected with vvΔE3L-Rev, vvΔE3L, vvE3LΔN, or vvE3LΔC25 at an MOI of 5 and collected at 6 hpi. The expression of E3 and β-actin was determined by Western blotting. (C) Confluent HeLa cells were mock infected or infected with vvΔE3L-Rev, vvΔE3L, vvE3LΔN, or vvE3LΔC25 at an MOI of 5. Cells were collected at 12 hpi, and RNA was extracted for RT-PCR.