FIG. 2.

PKR, p38, and IPS-1 mediate the cytokine response to vvΔE3L. (A) Confluent HeLa cells in six-well plates were either mock infected or infected with vvΔE3L-Rev or vvΔE3L at an MOI of 5. Cells were collected at the times indicated. Selected wells were also treated with 50 μg/ml of araC 1 h prior to infection. Western blotting was performed to detect total and phosphorylated PKR and p38. (B) HeLa cells (1.5 × 10⁵) were seeded in six-well plates. Cells were then transfected with 100 nM PKR-, p38-, or IPS-1-specific siRNAs for 72 h. Western blotting was performed to determine the efficiency of the siRNA knockdown. (C) Cells were transfected as described above and then either mock infected or infected with vvΔE3L-Rev or vvΔE3L at an MOI of 5. Cells were collected at 12 hpi, and RNA was extracted for RT-PCR. Results are representative of three independent experiments.