FIG. 4.

E3 suppresses cytokine expression through NF-κB- and IRF3-mediated pathways. (A) HeLa cells (1.5 × 10⁵) were seeded in six-well plates. Cells were then transfected with 100 nM NF-κB p65-, NF-κB p50-, or IRF3-specific siRNAs for 72 h. Western blotting was performed to determine the efficiency of the siRNA knockdown. (B) Cells were transfected as described above and then either mock infected or infected with vvΔE3L-Rev or vvΔE3L at an MOI of 5. Cells were collected at 12 hpi, and RNA was extracted for RT-PCR. Results are representative of three independent experiments.