FIG. 2.

Biochemical analysis of the subcellular distribution of proteins involved in HRR. Lytic replication was induced in Tet-BZLF1/B95-8 cells with 2 μg/ml of doxycycline, and cells were harvested at the indicated h p.i. As described in Materials and Methods, whole-cell lysates (W), Triton X-100-extractable supernatants (S), and extracted nuclear fractions (P) were prepared. The extracted nuclei were further treated with DNase I to obtain DNase I-solubilized supernatants (S under DNase) and remaining insoluble nuclear pellets (P under DNase). These fractions were applied for immunoblotting analyses with the antibodies indicated on the right. Samples corresponding to 1.8 × 10⁴ cells were applied to each lane.