FIG. 7.

RNAi knockdown of RPA32 and Rad51 prevented viral genome synthesis. To examine the influence of Rad51 or RPA32 on EBV genome synthesis, 293/EBV cells were transfected with siRNA specific for Rad51, RPA32, or control GFP, respectively, and cultured for 24 h. Subsequently, cells were transfected with expression vector pBZLF1 and harvested 48 h after the induction of EBV lytic replication. (Top) Viral DNA synthesis was determined by slot blot assay as described in Materials and Methods. The means ± standard deviations of data from three independent experiments are shown. (Bottom) Cell lysates were analyzed by immunoblotting with antibodies to the indicated proteins.