FIG. 4.

Interaction of hRBL2 with viral proteins. (A) hRBL2 interacts with viral RNP complexes. The three viral polymerase proteins and c-Myc-tagged NP were expressed in 293T cells in the absence or presence of virus-like RNA (lanes 1 and 2). Nontagged NP was expressed as a negative control (lane 3). One day later, cell lysates were immunoprecipitated with anti-c-Myc beads. Western blot analysis was carried out with anti-hRBL2 and anti-c-Myc antibodies. EGFP expression in transfected cells indicates the functionality of c-Myc-tagged NP protein. (B) hRBL2 interacts with NP, PB2, and PB1. The three polymerase and NP proteins were individually tested for their interaction with hRBL2 as described above. α, anti; IP, immunoprecipitation.