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Exposure to the microtubule-destabilizing drug nocodazole abolished the filamentous vp13 pattern. HeLa cells were transiently transfected with the VenusC1-H3 plasmid and exposed to nocodazole on the next day after transfection. Using confocal microscopy, we found that microtubule depolymerization abolished the filamentous pattern of the vp13-Venus fusion protein (A), but it was recovered in culture 4 h after the drug was removed (B).
