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. 2009 Apr 22;83(13):6717–6726. doi: 10.1128/JVI.02213-08

FIG. 8.

FIG. 8.

Ras/MEK pathway controls the transcription of STAT2 mRNA. (A) Inhibition of MEK in RasV12 cells increases the expression of STAT2 mRNA. Vector control and RasV12 cells were treated with IFN-α (500 U/ml), U0126 (20 μM), SL327 (20 μM), or PD98059 (40 μM) for 6 h. Levels of STAT2, STAT1, OAS, and GAPDH mRNA were determined by RT-PCR. (B) Degradation of STAT2 mRNA in RasV12 cells is not altered by U0126 treatment. RasV12 cells were pretreated with 4 μM ActD for 1 h, followed by treatment with U0126 (20 μM). STAT2 and 18S mRNA levels were determined by RT-PCR (right) and quantitative RT-PCR (left). Data are means ± standard errors of the means from three independent experiments. (C) Protein synthesis is required for the derepression of STAT2 mRNA transcription. RasV12 cells were pretreated with 20 μg/ml CHX for 1 h and then treated with U0126 (20 μM) or IFN (500U/ml) for 6 h. STAT2, OAS, and GAPDH mRNA levels were determined by RT-PCR.