FIG. 6.

Results from single-peptide screening of overlapping peptides from NS1 demonstrate its immunogenicity in the primary CD4 response to influenza virus infection. Mice were infected intranasally with A/New Caledonia/20/99 virus, and CD4 T cells were isolated from the spleen 10 days postinfection. The numbers of IL-2-producing CD4⁺ T cells that are reactive to the test single 17-mer peptide are shown. The results shown are the averages of data from six independent experiments, with the standard errors of the means shown. The response to the immunodominant HA peptide (HA-75) is shown in gray.