FIG. 3.
Hey1 is degraded by a proteasome-dependent mechanism in the presence of RTA. HEK 293T cells were cotransfected with Hey1-Myc and RTA or RTAH145L in the absence and presence of the proteasome inhibitor MG132 (5 μM) added at 12 h posttransfection. Cell lysates were analyzed at 36 h posttransfection by immunoblotting using a Myc tag-specific monoclonal IgG to determine the levels of Hey1-Myc. RTA-specific polyclonal IgG was used to confirm expression of RTA and RTAH145L. An actin-specific monoclonal IgG was used as a loading control.