FIG. 7.

A26-A27 complex formation in cells infected with vE10Ri. (A) BSC40 cells were infected with vE10Ri, and cells were harvested at 24 h p.i. with 10% TCA precipitation (TCA ppt) and NEM as described in Materials and Methods. Protein samples were added with or without TCEP, boiled, and separated on gels using anti-A27 Ab. (B) Samples were loaded in the same order as that used for panel A and were probed with anti-A26 Ab. The asterisk represents the degraded A26 protein. (C) The growth of vE10Ri virus was regulated by IPTG. (D) Immunoblot analyses (IB) of total L1 protein (top) and the oxidized form of L1 protein (L1 ox). L1 red, reduced form of L1 protein. 2D5 MAb only recognized the oxidized form of L1R on nonreducing gels (24).