FIG. 5.
Germinal center B-cell analysis. (A) Mice were infected with the indicated virus and spleens were harvested at the indicated day postinfection. Germinal center B cells were defined as CD19+ CD95hi GL7hi. The top row shows representative flow plots of the total CD19-positive population, and the bottom row shows YFP-positive cells (black dots) overlaid onto the total CD19 population. The numbers in the top row represent the percentage of CD19-positive cells that are germinal center B cells, and the numbers in the bottom row represent the percentage of YFP-positive cells that are germinal center positive. (B) Percentage of total B cells that are germinal center positive. (C) Percentage of YFP-positive cells that are germinal center positive. (D) Representative flow plots showing the percentage of germinal center cells that are infected based on YFP expression. (E) Percentage of infected germinal center cells at the indicated day postinfection based on YFP expression. In panels B, C, and E, the solid bar represents the mean. The results are shown from two independent experiments with three to four mice per group.