FIG. 4.

HCV NS5A levels during second-phase IFN-α inhibition of sg1b RNA. At 5, 6, 7, and 8 days posttreatment, mock-treated cells (A) and sg1b cell cultures treated with 100 U/ml (B) and 250 U/ml (C) IFN-α were fixed and stained for HCV NS5A. Cell nuclei were stained by Hoechst dye. Shown are representative ×20 images taken at day 6 posttreatment. HCV NS5A is shown in red (Alexa 555), and nuclei are shown in blue (Hoechst). Bar, 50 μm.