FIG. 4.

Extracellular release of 3A-2 mutant poliovirus. HeLa cells were infected as described in the legend to Fig. 2 with wild-type and 3A-2 mutant viruses. (A) A time course of the amounts of 3A-2 extracellular virus in the medium of infected cells cultured in the presence or absence of 5 μM nocodazole. In independent experiments, the titers of intracellular (B) or extracellular (C) virus from cells infected with wild-type and 3A-2 mutant viruses in the presence and absence of 5 μM nocodazole as indicated are shown. Mean values and standard errors from triplicate experiments are provided.