Fig. 2.

Activation of myocardial phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling in WT and ERβKO hearts of both sexes following I/R. A: ablation of ERβ reduced PI3K activation in both sexes, but with relatively greater effect in female hearts. B: ERβKO decreased myocardial Akt activity only in female, not male, hearts. Representative immunoblots (2 lanes/group) are shown at left. Densitometry data [percentages of phosphorylated (p) to total (T) protein] are shown at right. Results are means ± SE. *P < 0.05; **P < 0.01 vs. corresponding WT. Experiments were repeated in 2 different sessions.